Journal: Frontiers in Cell and Developmental Biology

Article Title: A differentiation protocol for generating pancreatic delta cells from human pluripotent stem cells

doi: 10.3389/fcell.2024.1490040

Figure Lengend Snippet: Final concentration of differentiation factors.

Article Snippet: • Activin A (StemCell Technologies, cat. no. 78001) • CHIR99021 (Stemgent, cat. no. 04-0004-10) • FGF7 (StemCell, cat. no. 78046) • FGF2 (Med Chem Express, cat. no. HY-P7004) • Retinoic acid (Sigma, cat. no. R2625) • SANT-1 (Sigma, cat. no. S4572) • LDN193189 (Reprocell, cat. no. 40074) • PdBU (Millipore, cat. no. 524390) • ALK5i II (Cell Guidance Systems, cat. no. SM09-50) • XXI (Millipore, cat. no. 595790) • Betacellulin (Med Chem Express, cat. no. HY-P7005) • Heparin (Sigma, cat. no. H3149-500KU) • N-acetyl cysteine (Sigma, cat. no. A9165-100G) • R428 (Selleck, cat. no. S2841) • α-Tocopherol (Sigma, cat. no. T3251) • Zinc sulfate (Sigma, cat. no. Z0251) • PBS for dissolving factors (HyClone, cat. no. SH30256.01) • Dimethyl sulfoxide for dissolving factors (DMSO) (MilliporeSigma, cat. no. D4540-100ML)

Techniques: Concentration Assay

Journal: Molecules

Article Title: Quercitrin Stimulates Hair Growth with Enhanced Expression of Growth Factors via Activation of MAPK/CREB Signaling Pathway

doi: 10.3390/molecules25174004

Figure Lengend Snippet: Effect of quercitrin on the protein expression of bFGF and KGF in cultured hDPCs. The DPCs were treated with quercitrin at concentrations of 1, 10, 100 nM and 1 µM for 24 h. Whole cell lysates ( a , c ) and culture medium ( b , d ) of each cultured DPCs were analyzed by ELISA to determine the levels of bFGF (a,b) and KGF (c,d). N.T, non-treated control. Significantly different compared with N.T (* p < 0.05, ** p < 0.01, *** p < 0.001).

Article Snippet: DPCs were treated with various concentrations of quercitrin for 24 h. The cells and culture medium were collected and the amounts of bFGF and KGF were measured using human bFGF and human KGF DuoSet ELISA (R&D systems) kits, according to the manufacturer’s instruction.

Techniques: Expressing, Cell Culture, Enzyme-linked Immunosorbent Assay, Control

Journal:

Article Title: Induction of Keratinocyte Growth Factor 1 Expression by Lipopolysaccharide Is Regulated by CD-14 and Toll-Like Receptors 2 and 4

doi: 10.1128/IAI.70.12.6541-6548.2002

Figure Lengend Snippet: LPS purified from E. coli significantly induces gingival fibroblast expression of KGF-1 protein in the presence or absence of serum. Gingival fibroblasts were cultured to quiescence in α-MEM supplemented with 1% FBS. Various concentrations of LPS (A) and recombinant human sCD14 (B), with or without LPS (50 ng/ml), were added to serum-free and 1% FBS-supplemented α-MEM. A sandwich ELISA was used to analyze KGF-1 protein levels in 24-h conditioned medium. Mean ± standard deviation; n = 4.

Article Snippet: Plates were coated with the capturing anti-human KGF-1 monoclonal antibody MAB251 (R&D Systems Inc.) at 1.0 μg/ml, washed, and blocked (2% bovine serum albumin [BSA], 5% sucrose, 0.05% NaN 3 ).

Techniques: Purification, Expressing, Cell Culture, Recombinant, Sandwich ELISA, Standard Deviation

Journal:

Article Title: Induction of Keratinocyte Growth Factor 1 Expression by Lipopolysaccharide Is Regulated by CD-14 and Toll-Like Receptors 2 and 4

doi: 10.1128/IAI.70.12.6541-6548.2002

Figure Lengend Snippet: Addition of CD14-blocking antibody inhibits LPS stimulation of KGF-1 protein expression. Gingival fibroblasts were brought to quiescence in α-MEM supplemented with 1% FBS. In replicate wells, the following treatment groups were tested: α-MEM with 1% FBS (control), α-MEM with 1% FBS plus 50 ng of E. coli LPS (LPS) per ml or first pretreatment for 30 min with 0.5 μg of anti-CD14 blocking antibody per ml with α-MEM and 1% FBS prior to the addition of 50 ng of LPS per ml. KGF-1 protein in the 24-h conditioned medium was measured by a sandwich ELISA. Mean ± standard deviation; n = 4.

Article Snippet: Plates were coated with the capturing anti-human KGF-1 monoclonal antibody MAB251 (R&D Systems Inc.) at 1.0 μg/ml, washed, and blocked (2% bovine serum albumin [BSA], 5% sucrose, 0.05% NaN 3 ).

Techniques: Blocking Assay, Expressing, Sandwich ELISA, Standard Deviation

Journal:

Article Title: Induction of Keratinocyte Growth Factor 1 Expression by Lipopolysaccharide Is Regulated by CD-14 and Toll-Like Receptors 2 and 4

doi: 10.1128/IAI.70.12.6541-6548.2002

Figure Lengend Snippet: LPS downregulates cell membrane expression of TLR2 and TLR4. Gingival fibroblasts were treated with either E. coli or P. gingivalis (P. ging) LPS (50 ng/ml), collected at various times, fixed, stained without permeabilization with either anti-TLR2 (TLR2) or anti-TLR4 (TLR4) monoclonal antibody, and then stained with an alexa 488 nm-conjugated secondary antibody prior to flow cytometry analysis. Mean ± standard deviation; n = 4. (B) Preincubation of cultures with TLR2 and TLR4 blocking antibodies inhibited E. coli LPS stimulation of KGF-1 protein expression. Culture groups were treated with α-MEM with 1% FBS (control) or α-MEM with 1% FBS and 50 ng of LPS (LPS) per ml or first pretreated for 30 min with either 0.5 μg of anti-TLR2 (LPS+aT2) or TLR4 (LPS+aT4) blocking antibody per ml with α-MEM plus 1% FBS prior to the addition of LPS at 50 ng/ml. A sandwich ELISA was used to measure KGF-1 protein expression in 24-h conditioned medium. Mean ± standard deviation; n = 4.

Article Snippet: Plates were coated with the capturing anti-human KGF-1 monoclonal antibody MAB251 (R&D Systems Inc.) at 1.0 μg/ml, washed, and blocked (2% bovine serum albumin [BSA], 5% sucrose, 0.05% NaN 3 ).

Techniques: Membrane, Expressing, Staining, Flow Cytometry, Standard Deviation, Blocking Assay, Sandwich ELISA

Journal:

Article Title: Induction of Keratinocyte Growth Factor 1 Expression by Lipopolysaccharide Is Regulated by CD-14 and Toll-Like Receptors 2 and 4

doi: 10.1128/IAI.70.12.6541-6548.2002

Figure Lengend Snippet: LPS induction of KGF-1 protein expression is regulated by the transcription factors AP-1 and NF-κB. Quiescent gingival fibroblasts in α-MEM with 1% FBS were preincubated for 30 min with increasing concentrations of the transcription factor inhibitors curcumin (A) and PDTC (B) prior to the addition of E. coli LPS (50 μg/ml). Conditioned medium was collected at 24 h, and the KGF-1 protein level was assayed by a sandwich ELISA. The dotted line represents the KGF-1 protein expression of the negative control (1% serum alone). RNA from cultures was isolated at 6 h for Northern analysis (C). Lanes: 1, negative control (1% serum alone); 2, E. coli LPS at 50 ng/ml; 3, LPS plus PDTC at 2 nM; 4, LPS plus PDTC at 4 nM; 5, LPS plus PDTC at 6 nM; 6, LPS plus curcumin at 5 μM; 7, LPS plus curcumin at 10 μM; 8, LPS plus curcumin at 30 μM.

Article Snippet: Plates were coated with the capturing anti-human KGF-1 monoclonal antibody MAB251 (R&D Systems Inc.) at 1.0 μg/ml, washed, and blocked (2% bovine serum albumin [BSA], 5% sucrose, 0.05% NaN 3 ).

Techniques: Expressing, Sandwich ELISA, Negative Control, Isolation, Northern Blot